RNASE1 Rabbit Polyclonal Antibody

CAT#: R1109BS

RNASE1 rabbit polyclonal antibody, Biotin, Purified

Product Images

Western blot of rabbit anti-Ribonuclease A antibody biotin conjugated. Lane 1: Ribonuclease A (bovine pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A antibody biotin conjugated at 1/1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band (s): Possible dimerization.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western Blot : 1/500 - 1/2,500.
  Immunoprecipitation: 1/100.
  ELISA : 1/2,500 - 1/10,000.
  This product has been assayed against 1.0 µg of Ribonuclease A [bovine pancreas] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/13,000 is suggested.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Ribonuclease A from bovine pancreas
• Specificity: This antibody detects bovine RNASE1. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Ribonuclease A [bovine pancreas].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Database Link:
  Entrez Gene 282340 Bovine
• Synonyms: Ribonuclease 1, RNase 1, Ribonuclease A, RNase A, RIB1, RIB-1, RNS1, Ribonuclease pancreatic