PRSS1 Rabbit Polyclonal Antibody

CAT#: R1113HRPS

PRSS1 rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/14,000.
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Trypsin from bovine pancreas
• Specificity: This antibody detects bovine trypsin. Cross reactivity from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified trypsin [bovine pancreas].
• Formulation: 0.02 M Potassium pPhosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Database Link:
  Entrez Gene 615026 Bovine
• Background: Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.
• Synonyms: TRP1, TRY1, TRYP1, Trypsin I, Cationic trypsinogen, Beta-trypsin, Serine protease 1