KTI3 Rabbit Polyclonal Antibody

CAT#: R1114HRPS

KTI3 rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/1,000-1/5,000.
  ELISA: 1/10,000-1/40,000.
  Antibody has been assayed against 1.0 µg of Trypsin inhibitor [lima bean] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
  
• Reactivities: Lima Bean
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Trypsin inhibitor from lima bean
• Specificity: This antibody detects lima bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [lima bean].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
  Label: HRP
  State: Lyophilized IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP