KTI3 Rabbit Polyclonal Antibody

CAT#: R1115HRPS

KTI3 rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/1,000-1/5,000.
  ELISA: 1/2,000-1/10,000.
  Immunohistochemistry: 1/200-1/1,000.
• Reactivities: Soy Bean
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Trypsin inhibitor from soybean
• Specificity: This antibody detects soybean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [soybean].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
  Label: HRP
  State: Lyophilized IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-trypsin inhibitor antibody is ideal for investigators involved in cell signaling, biochemistry and signal transduction research.