Urease Rabbit Polyclonal Antibody

CAT#: R1117HRPS

Urease rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Urease [jack bean] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2,000 of the reconstitution concentration is suggested.
• Reactivities: Jack Bean
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Urease from jack bean
• Specificity: This antibody detects jack bean urease. Cross reactivity against urease from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified urease [jack bean].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunogen and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Synonyms: Urea amidohydrolase