ABC3735 Goat Polyclonal Antibody

CAT#: R1118BS

ABC3735 goat polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  ELISA: 1/1000-1/2500;
  This antibody has been assayed against 1.0 ug of uricase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.
  Immunoprecipitation: 1:100.
• Reactivities: Bacillus sp.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Uricase from Bacillus species
• Specificity: This Antibody detects Uricase [Bacillus species]. Cross reactivity against uricase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified uricase [Bacillus species].
  
  
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Background: Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH's.
• Synonyms: URIC, URO, UOX, EC 1.7.3.3, Urate oxidase