ABC3735 Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | ELISA: 1/1000-1/2500. Immunoprecipitation: 1/100. Western blot: 1/500-1/1000. |
Reactivities | Bacillus sp. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Uricase from Bacillus species |
Specificity | This product detects Uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/mL BSA - immunoglobulin and protease free Preservative: 0.01% Gentamicin sulfate. (Do NOT add Sodium azide!) |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Background | Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. |
Synonyms | URIC, URO, UOX, EC 1.7.3.3, Urate oxidase |
Reference Data |
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