ABC3735 Goat Polyclonal Antibody

CAT#: R1118HRPS

ABC3735 goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 1/1000-1/2500.
  Immunoprecipitation: 1/100.
  Western blot: 1/500-1/1000.
• Reactivities: Bacillus sp.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Uricase from Bacillus species
• Specificity: This product detects Uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/mL BSA - immunoglobulin and protease free
  Preservative: 0.01% Gentamicin sulfate. (Do NOT add Sodium azide!)
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
• Synonyms: URIC, URO, UOX, EC 1.7.3.3, Urate oxidase