XDH Rabbit Polyclonal Antibody

CAT#: R1119HRPS

XDH rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
  Western Blot: 1:500 - 1:5,000
  Immunoprecipitation: 1:100
  ELISA: 1:5,000 - 1:20,000.
  This product has been assayed against 1.0 µg of Xanthine oxidase [bovine] in a standard capture ELISA using ABTSas a substrate for 30 minutes at room temperature. A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Xanthine oxidase (XDH) from bovine buttermilk
• Specificity: This antibody detects bovine XDH. Cross reactivity against xanthine oxidase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine oxidase [bovine].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% Gentamicin sulfate (Do Not add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Database Link:
  Entrez Gene 280960 Bovine
• Synonyms: Xanthine dehydrogenase/oxidase