ACHE Goat Polyclonal Antibody

CAT#: R1148HRPS

ACHE goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 1/5,000-1/20,000.
  This antibody has been assayed against 1.0 µg of acetylcholinesterase [bovine erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 of the reconstitution concentration is suggested.
  Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
• Reactivities: Bovine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Acetylcholinesterase from bovine erythrocytes
• Specificity: This antibody detects bovine acetylcholinesterase. Cross reactivity against acetylcholinesterase from other sources is unknown.
  Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified acetylcholinesterase [bovine erythrocytes].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Database Link:
  Entrez Gene 540446 Bovine
• Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase