ACHE Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | ELISA: 1/5,000-1/20,000. This antibody has been assayed against 1.0 µg of acetylcholinesterase [bovine erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 of the reconstitution concentration is suggested. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. |
Reactivities | Bovine |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Acetylcholinesterase from bovine erythrocytes |
Specificity | This antibody detects bovine acetylcholinesterase. Cross reactivity against acetylcholinesterase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified acetylcholinesterase [bovine erythrocytes]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent) |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Gene Name | - |
Database Link | |
Synonyms | AChE, Acetylcholine Esterase, Acetylcholine-Esterase |
Reference Data |
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