Glucoamylase 1 Goat Polyclonal Antibody

CAT#: R1149HRPS

Glucoamylase 1 goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/1,000-1/5,000.
  ELISA: 1/10,000-1/40,000.
  This product has been assayed against 1.0 µg of Glucoamylase [Rhizopus] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested.
• Reactivities: Rhizopus
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glucoamylase from Rhizopus
• Specificity: This antibody detects Rhizopus Glucoamylase. Cross reactivity against Glucoamylase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Glucoamylase from Rhizopus.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Glucoamylase is an important industrial enzyme used in saccharification steps in both starch enzymatic conversion and in alcohol production. The catalytic domain degrades oligosaccharides from the non reducing end, releasing glucose, and the starch domain binds the enzyme to raw starch and to the cell wall.
• Synonyms: Gluc 1