Maltose Phosphorylase Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. |
Reactivities | Escherichia coli |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Maltose Phosphorylase from E.coli |
Specificity | This antibody detects Maltose Phosphorylase [E.coli]. Cross reactivity against Maltose Phosphorylase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Maltose Phosphorylase [E.coli]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Background | Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism. |
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