wecC Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Western blot: 1/500-1/5,000 Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of N-Acylmannosamine-1-Dehydrogenase [Pseudomonas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | N-Acyl Mannosamine-1-dehydrogenase [E.coli expressed Pseudomonas recombinant sequence]. |
Specificity | This antibody detects Pseudomonas N-Acylmannosamine-1-dehydrogenase. Cross reactivity against N-Acylmannosamine-1-dehydrogenase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified N-Acylmannosamine-1-dehydrogenase [Pseudomonas]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Background | N acylmannosamine 1 dehydrogenase belongs to the short chain dehydrogenases/ reductases family (SDR). It acts on acetyl D mannosamine and glycolyl D mannosamine. |
Synonyms | wecC, rffD, b3787, JW5599 |
Reference Data |
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