Pyranose Oxidase Goat Polyclonal Antibody

CAT#: R1156HRPS

Pyranose Oxidase goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot : 1/500 - 1/5,000.
  Immunoprecipitation: 1/100.
  ELISA : 1/5,000 - 1/20,000:
  This product has been assayed against 1.0 µg of Pyranose oxidase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
• Reactivities: Escherichia coli
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Pyranose oxidase from E.coli
• Specificity: This antibody detects Pyranose oxidase from E. coli. Cross reactivity against Pyranose oxidase from other microbes is known but not tested.
  Iimmunoelectrophoresis give a single precipitin arc against anti-biotin anti-goat serum as well as purified and partially purified Pyranose oxidase [E.coli].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP