Pyranose Oxidase Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Western blot : 1/500 - 1/5,000. Immunoprecipitation: 1/100. ELISA : 1/5,000 - 1/20,000: This product has been assayed against 1.0 µg of Pyranose oxidase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested. |
Reactivities | Escherichia coli |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Pyranose oxidase from E.coli |
Specificity | This antibody detects Pyranose oxidase from E. coli. Cross reactivity against Pyranose oxidase from other microbes is known but not tested. Iimmunoelectrophoresis give a single precipitin arc against anti-biotin anti-goat serum as well as purified and partially purified Pyranose oxidase [E.coli]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Reference Data |
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