pgmB Goat Polyclonal Antibody

CAT#: R1157BS

pgmB goat polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3000.
  ELISA: 1/5,000-1/25,000 dilution suggested to begin optimization.
  This product has been assayed against 1.0 µg of Beta-phosphoglucomutase [Lactococcus lacti] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested.
• Reactivities: Lactococcus lacti
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Beta-phosphoglucomutase (PGMB) from Lactococcus lacti
• Specificity: This antibody detects Beta-phosphoglucomutase from Lactococcus lacti. Cross reactivity against Beta-phosphoglucomutase from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified beta-phosphoglucomutase [Lactococcus lacti].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Synonyms: Beta-phosphoglucomutase, Beta-PGM, Phosphoglucomutase beta, Glucose Phosphomutase