c-Myc (MYC) Rabbit Polyclonal Antibody

CAT#: R1182HRP

c-Myc (MYC) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10,000-1/50,000.
  Western Blot: 1/2,000 - 1/5,000.
  Immunohistochemistry: 1/500-1/2000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
• Specificity: This antibody is directed against human c-myc.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Gentamicin Sulfate as perservative
  Label: HRP
  State: Lyophilized
  Label: Horseradish Peroxidase
  Absorption emission: 770 nm / 794 nm
  Molar radio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: HRP
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch
• Gene Name: Homo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC)
• Database Link:
  Entrez Gene 4609 Human
• Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
• Synonyms: myc tag, myc-tag, c-myc tag

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway