Ferret IgG (gamma chain specific) Goat Polyclonal Antibody

CAT#: R1313B

Ferret IgG (gamma chain specific) goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western Blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Note: This product has been assayed against 1.0 µg of Ferret IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1/5,000 to 1/20,000 of the concentration is suggested for this product.
• Reactivities: Felinerret
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Ferret IgG γ chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgG and Ferret Serum.
  No reaction was observed against Ferret IgA or Ferret IgM.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Synonyms: Ferret Immunoglobulin G