Ferret IgA, IgG, IgM (H+L chain) Goat Polyclonal Antibody

CAT#: R1316B

Ferret IgA, IgG, IgM (H+L chain) goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (WB 1/2,000-1/10,000) ELISA ( 1/20,000-1/100,000), Immunohistochemistry (1/1000-1/5000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Ferret IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1/1,000 to 1/4,000 is suggested for this product.
• Reactivities: Felinerret
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Ferret IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity hromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.
  This product is suitable for the detection of all Ferret immunoglobulin classes, isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Synonyms: Ferret IgA, Ferret IgG, Ferret IgM