Human Kappa Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1401AP

Human Kappa Light Chain (F(ab)2 Fragment) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Imunoblotting (Wstern or Dt blot), ELISA and Imunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human kappa chain.
• Specificity: This product was prepared from monospecific antiserum by Imunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Imunoelectrophoresis resulted in a single precipitin arc against anti- Alkaline Phosphatase and anti-Goat Serum.
  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
  Label: AP
  State: Liquid (sterile filtered) F(ab')2 fragments.
  Stabilizer:
  10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
  
  Preservative:
  0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP