Human Lambda Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1402T

Human Lambda Light Chain (F(ab)2 Fragment) goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human λ (lambda chain).
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.
  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilized F(ab')2 fragments.
  Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 4.0 moles TRITC per mole of Goat IgG F(ab')2).
• Reconstitution Method: Restore with 0.4 ml of deionized water (or equivalent).
• Concentration: 2.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: TRITC