Human Lambda Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody
CAT#: R1402TR
Human Lambda Light Chain (F(ab)2 Fragment) goat polyclonal antibody, Texas Red
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Specifications
Product Data | |
Recommended Dilution | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human lambda light chain |
Specificity | This antibody detects Human Lambda Light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. |
Formulation | 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer; 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer; 0.01% (w/v) Sodium Azide as preservative Label: Texas Red State: Lyophilized F(ab)2 Fragment Label: Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 1.6 moles Texas RedTM per mole of Goat IgG F(ab')2 |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). Dilute only prior to immediate use. |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
Conjugation | Texas Red |
Reference Data |
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