Cyanine Dyes Sheep Polyclonal Antibody

CAT#: R1460B

Cyanine Dyes sheep polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended dilutions: This product has been assayed against 1.0 ug of Cyanine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum and cyanine conjugated IgG.
  Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% Sodium Azide as preservative and 10 mg/ml BSA as stabilzer, IgG and Protease free.
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecule per sheep IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: Biotin