Cyanine Dyes Sheep Polyclonal Antibody

CAT#: R1460P

Cyanine Dyes sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western blot: 1/1,000-1/5,000.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and Cyanine conjugated IgG.
  Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride containing 0.01% (w/v) Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) Ig fraction.
• Concentration: 1.06 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using a Cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.