Apc11 (ANAPC11) Rabbit Polyclonal Antibody

CAT#: R1503

Apc11 (ANAPC11) rabbit polyclonal antibody, Serum


USD 530.00

3 Days*

Size
    • 100 ul

Product Images

Specifications

Product Data
Applications Assay, ELISA, IP, WB
Recommended Dilution Western blot (1:500-1:1,000).
ELISA (1:2,000-1:10,000).
Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen This APC11 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This antibody reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
Formulation State: Serum
State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative.
Concentration 85 mg/ml (by Refractometry)
Purification Delipidation and defibrination.
Background APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Synonyms Anaphase-promoting complex subunit 11, HSPC214
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.