TIP120A (CAND1) Rabbit Polyclonal Antibody

CAT#: R1507

TIP120A (CAND1) rabbit polyclonal antibody, Serum

Product Images

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF (SKP1/cullin-1/F-box protein) /CBC (cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP (ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S. Jentsch, see references above.

Specifications

Product Data

• Applications: Assay, ELISA, IP, WB
• Recommended Dilution: Western blot (1:500-1:1,000).
  Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle and heart tissue.
  ELISA: (1:2,000-1:10,000).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
• Specificity: This antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
  It reacts with human, rat and mouse CAND2/TIP120B.
  Cross reactivity does occur with human, rat and mouse CAND1/TIP120A.
  Cross reactivity with CAND2 from other sources is not known.
• Formulation: State: Serum
  State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative.
• Concentration: 85 mg/ml (by Refractometry)
• Purification: Delipidation and defibrination.
• Database Link:
  Entrez Gene 55832 Human
• Background: CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.
• Synonyms: KIAA0829, TIP120, TIP120A, p120 CAND1