Biotin Goat Polyclonal Antibody

CAT#: R1615HRP

Biotin goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/140,000.
  Western blot: 1/3000-1/15,000.
  Immunohistochemistry: 1/1000-1/5000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Biotin coupled to sepharose beads.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Biotinylated Bovine Serum Albumin and biotinylated IgG.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H
• Note: Do Not Add Sodium Azide!