Biotin Goat Polyclonal Antibody

CAT#: R1615HRP

Biotin goat polyclonal antibody, HRP


USD 660.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/140,000. 
Western blot: 1/3000-1/15,000. 
Immunohistochemistry: 1/1000-1/5000.
Host Goat
Clonality Polyclonal
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Biotin coupled to sepharose beads.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Biotinylated Bovine Serum Albumin and biotinylated IgG.
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Label: HRP
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Preservative: 0.01% (w/v) Gentamicin Sulfate
Label: Horseradish Peroxidase
Reconstitution Method Restore with 1.0 ml of deionized water or equivalent.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography
Conjugation HRP
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms Vitamin B7, Vitamin H
Note Do Not Add Sodium Azide!
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.