Glutamine Synthetase (GLUL) Rabbit Polyclonal Antibody

CAT#: RA25062-100

Glutamine Synthetase (GLUL) rabbit polyclonal antibody

Product Images

Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction [using ABC kit and visualization with DAB product in F]. Nuclei in some immunofluorescence experiments (A-D) were stained with DAPI (shown in cyan), and with nuclear fast red in E and F. On inspection at low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL). No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). The pattern of staining observed in all experiments is typical of Müller cells. This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).

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  Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction [using ABC kit and visualization with DAB product in F]. Nuclei in some immunofluorescence experiments (A-D) were stained with DAPI (shown in cyan), and with nuclear fast red in E and F. On inspection at low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL). No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). The pattern of staining observed in all experiments is typical of Müller cells. This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).

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  Western blot analysis of glutamine synthase. 40ug of lysates from mouse (lane M), rat (lane R), pig (lane P), bovine (lane B), or human (lane Hu) retina were probed. A 34 kDa band was identified.

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  Western blot analysis of glutamine synthase. 40ug of lysates from mouse (lane M), rat (lane R), pig (lane P), bovine (lane B), or human (lane Hu) retina were probed. A 34 kDa band was identified.

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: State: Serum
• Conjugation: Unconjugated
• Gene Name: Homo sapiens glutamate-ammonia ligase (GLUL), transcript variant 2
• Database Link:
  Entrez Gene 14645 MouseEntrez Gene 24957 RatEntrez Gene 2752 Human
  UniProt ID P15104
• Synonyms: GS, GLUL, GLNS, Glutamate decarboxylase

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism