Cd3e Rat Monoclonal Antibody [Clone ID: KT3]

CAT#: SM003PT

Cd3e rat monoclonal antibody, clone KT3, Purified

Specifications

Product Data

• Clone Name: KT3
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 1x10e6 cells. For optimal staining incubations should be performed at room temperature.
  Immunohistochemistry on Frozen Sections.
  For optimal staining incubations should be performed at room temperature.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
• Specificity: This antibody recognizes the Mouse CD3 antigen, which is expressed by mature T cells.
  The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Database Link:
  Entrez Gene 12501 Mouse
• Background: Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Synonyms: T3/Leu-4