Cd4 Rat Monoclonal Antibody [Clone ID: YTS191.1]

CAT#: SM005PS

Cd4 rat monoclonal antibody, clone YTS191.1, Purified

Product Images

Staining of mouse spleen cells with Rat anti Mouse CD4 (SM005PS)

Specifications

Product Data

• Clone Name: YTS191.1
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 10⁶ cells.
  Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to Formaldehyde Fixation and tissue processing. The use of Acetone fixation for frozen sections is recommended.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes.
  This clone exhibits depleting activity when used in vivo (Azide Free CD4 Antibody Cat.-No SM005LE is recommended for this purpose).
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 12504 Mouse
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3