Cd40 Rat Monoclonal Antibody [Clone ID: 3/23]

CAT#: SM008R

Cd40 rat monoclonal antibody, clone 3/23, PE

Product Images

Staining of Mouse spleen with Rat Anti Mouse CD40-RPE.

Specifications

Product Data

• Clone Name: 3/23
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of diluted antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
• Specificity: This antibody recognises CD40 and stains most mature mouse B cells. T cells are unlabelled.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water:
  SM008R with 1.0 ml.
  SM008RT with 0.25 ml
  SM008RX with 5.0 ml
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Database Link:
  Entrez Gene 21939 Mouse
• Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
• Synonyms: TNFRSF5, CDw40, Bp50