Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

CAT#: SM011FSX

Cd8a rat monoclonal antibody, clone KT15, FITC

Specifications

Product Data

• Clone Name: KT15
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T cell clone, C6. Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
• Specificity: This antibody reacts with a non-polymorphic epitope on the CD8 alpha chain. Stains Ly2.1 and Ly2.2 cells.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Purification: Affinity chromatography on Protein A
• Conjugation: FITC
• Database Link:
  Entrez Gene 12525 Mouse
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL