Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

CAT#: SM011P

Cd8a rat monoclonal antibody, clone KT15, Purified

Specifications

Product Data

• Clone Name: KT15
• Applications: FC, IF, IHC
• Recommended Dilution: Flow cytometry: 1/10 - 1/50, use 10 µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T cell clone, C6. Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
• Specificity: This antibody recognises mouse Ly2.1 and Ly2.2 cells; reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.
• Formulation: PBs, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein A
• Gene Name: Mus musculus CD8 antigen, alpha chain (Cd8a), transcript variant 1
• Database Link:
  Entrez Gene 12525 Mouse
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL