Cd8a Rat Monoclonal Antibody [Clone ID: KT15]
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Specifications
Product Data | |
Clone Name | KT15 |
Applications | FC, IF, IHC |
Recommended Dilution | Flow cytometry: 1/10 - 1/50, use 10 µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections. |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | T cell clone, C6. Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line. |
Specificity | This antibody recognises mouse Ly2.1 and Ly2.2 cells; reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic. |
Formulation | PBs, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG |
Concentration | 1.0 mg/ml |
Purification | Affinity chromatography on Protein A |
Gene Name | Mus musculus CD8 antigen, alpha chain (Cd8a), transcript variant 1 |
Database Link | |
Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
Synonyms | CD8 alpha chain, CD8A, MAL |
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