Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

CAT#: SM011R

Cd8a rat monoclonal antibody, clone KT15, PE

Product Images

Staining of Mouse spleen cells with Rat Anti Mouse CD8 ALPHA-RPE

Specifications

Product Data

• Clone Name: KT15
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T cell clone, C6.
  Spleen cells from a Spraque Dawley rat immunised with the C6 T cell clone were fused with cells of the NSO mouse myeloma cell line.
• Specificity: This antibody reacts to Ly2.1 and Ly2.2 cells.
  Reacts with the CD8 alpha chain (non polymorphic).
  Weakly cytotoxic.
• Formulation: PBS containing 0.09% Sodium Azide as presrevative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water:
  SM011R with 1.0 ml
  SM011RT with 0.25 ml
  SM011RX with 5.0 ml
• Conjugation: PE
• Database Link:
  Entrez Gene 12525 Mouse
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL