CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014P

CD11b rat monoclonal antibody, clone M1/70.15, Purified

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: 1/50.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: Clone M1/70.15 has been reported to be suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on Formalin Fixed tissue (Ref.4)
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice.
  Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
• Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).
  Clone M1/70.15 has been reported to block iC3b binding to its receptor (Ref.3). SM014A is recommended for this purpose.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Ion Exchange Chromatography
• Background: CD11b is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1