CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014RT

CD11b rat monoclonal antibody, clone M1/70.15, PE

Product Images

Staining of total mouse peritoneal exudate cells demonstrating labelling of Macrophages with Rat anti Mouse CD11b-RPE

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice.
  Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
• Specificity: This antibody recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).
  Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose.
  Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water.
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Background: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages.
  CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
  Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1