Cd4 (non-polymorphic epitope) Rat Monoclonal Antibody [Clone ID: YTS177.9]

CAT#: SM019R

Cd4 (non-polymorphic epitope) rat monoclonal antibody, clone YTS177.9, PE

Specifications

Product Data

• Clone Name: YTS177.9
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse spleen cells.
  Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
• Specificity: This antibody reacts with the CD4 antigen; non polymorphic epitope.
  The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance. We recommend SM019A for such studies.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water.
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Database Link:
  Entrez Gene 12504 Mouse
• Background: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
  Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
  Intracellular ligands: p56Lck
  CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Synonyms: T-cell surface antigen T4/Leu-3