Cd28 Hamster Monoclonal Antibody [Clone ID: 37.51.1]

CAT#: SM023P

Cd28 hamster monoclonal antibody, clone 37.51.1, Purified

Specifications

Product Data

• Clone Name: 37.51.1
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry (use 5 µl of neat antibody to label 10e6 cells in 100 µl).
  Immunoprecipitation.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Mouse T lymphoma EL-4 cells
• Specificity: This antibody reacts with mouse CD28 found predominantly on T cell subsets and activated B cells.
• Formulation: PBS containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 0.2 mg/ml
• Purification: Affinity chromatography on Protein A
• Gene Name: CD28 antigen
• Database Link:
  Entrez Gene 12487 Mouse
• Background: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
• Synonyms: TP44