Msr1 Rat Monoclonal Antibody [Clone ID: 2F8]

CAT#: SM029P

Msr1 rat monoclonal antibody, clone 2F8, Purified

Product Images

Staining of J774 cell line with Rat Anti Mouse CD204.

Specifications

Product Data

• Clone Name: 2F8
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western blot: This antibody recognizes CD204 in J774 cells under non-reduced conditions only.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Mouse, Porcine
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Raw 264 cell line.
• Specificity: This antibody recognizes the Murine scavenger receptor class A (SRA), type I and II, also known as CD204.
  Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. We recommend the use of SM029LE for this purpose.
  Recent research shows that clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 Mouse strain.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 20288 Mouse
• Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions.
• Synonyms: MSR1, SCARA1