CD45 / LCA Rat Monoclonal Antibody [Clone ID: YW62.3]

CAT#: SM032PT

CD45 / LCA rat monoclonal antibody, clone YW62.3, Purified


USD 190.00

5 Days*

Size
    • 25 ug

Product Images

Specifications

Product Data
Clone Name YW62.3
Applications FC, IHC, IP
Recommended Dilution Flow cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen Mouse spleen cells.
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity This antibody recognises the CD45 cell surface antigen, a 180-220kD glycoprotein expressed by all leucocytes.
Some reactivity with human tissue has been observed.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration 1.0 mg/ml
Purification Affinity Chromatography on Protein G
Background CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.