CD45 / LCA Rat Monoclonal Antibody [Clone ID: RA3-6B2]

CAT#: SM034LE

CD45 / LCA rat monoclonal antibody, clone RA3-6B2, Low Endotoxin

Product Images

Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) PE.

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  Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) APC.

Specifications

Product Data

• Clone Name: RA3-6B2
• Applications: FC, FN, IF, IHC, IP
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Immunohistochemistry on paraffin sections.
  Immunohistochemistry on frozen sections.
  Immnocytochemistry.
  Functional application: modulation of B cell responses.
• Reactivities: Feline, Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
• Specificity: This antibody recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells.T cells detected by this antibody are supposed to be in activated state.
• Formulation: Phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-G affinity chromatography (> 95% pure by SDS-PAGE)
• Background: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200