Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

CAT#: SM038P

Integrin alpha 6 (ITGA6) rat monoclonal antibody, clone NKI-GoH3, Purified

Product Images

Staining of human peripheral blood lymphocytes with Rat Anti Human/Mouse CD49f Antibody

Specifications

Product Data

• Clone Name: NKI-GoH3
• Applications: FC, IHC, IP
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Recommended Positive Control: Human Tonsil.
• Reactivities: Canine, Human, Monkey, Mouse, Porcine
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
• Specificity: The monoclonal antibody is directed against the CDw49f-antigen. This monoclonal NKI-GoH3 antibody reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts. Platelet VLA-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by GoH3.
  Studies suggest that Clone NKI-GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (See Sonnenberg, A. et al. for details).
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 6
• Database Link:
  Entrez Gene 3655 Human
• Background: CDw49f-antigen is a 120kD, (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP 11a or VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.
• Synonyms: Integrin alpha-6, VLA-6, VLA6