Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

CAT#: SM038R

Integrin alpha 6 (ITGA6) rat monoclonal antibody, clone NKI-GoH3, PE

Product Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN/MOUSE CD49f (SM038R).

Specifications

Product Data

• Clone Name: NKI-GoH3
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 platelets in 100µl.
• Reactivities: Canine, Human, Monkey, Mouse, Porcine
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
• Specificity: This antibody recognises CD49F (VLA-6 alpha chain). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin. We recommend the use of SM038Afor this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 6
• Database Link:
  Entrez Gene 3655 Human
• Background: CD49F (VLA-6 alpha chain)is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1).
• Synonyms: Integrin alpha-6, VLA-6, VLA6