Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

CAT#: SM049R

Thy1 mouse monoclonal antibody, clone OX-7, PE

Specifications

Product Data

• Clone Name: OX-7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
  This product is routinely tested in flow cytometry on Rat thymocytes.
• Reactivities: Guinea Pig, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat Thy 1 antigen.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognizes CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
  Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy.
  Affinity Constant of the Fab' of MRC OX-7 for:
  Rat: 3 x 10⁹m^-1
  Mouse: 3 x 10⁸m^-1
  Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using Fluorescence Microscopy, See Ref.5 for details.
  This clone has been reported to induce glomerular nephritis in Wistar rats, See Ref.8 for details.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA and 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water.
• Purification: Ion Exchange Chromatography
• Conjugation: PE
• Database Link:
  Entrez Gene 21838 Mouse
• Background: CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
• Synonyms: Thy-1, THY1, CDw90