Siglec1 Rat Monoclonal Antibody [Clone ID: MOMA-1]

CAT#: SM066

Siglec1 rat monoclonal antibody, clone MOMA-1, Supernatant

Specifications

Product Data

• Clone Name: MOMA-1
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: 1/10.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stromal (reticular) elements from spleen.
• Specificity: This antibody is specific for CD169, also known as Sialoadhesin.
  It reacts with a subpopulation of mature resident tissue macrophages.
  No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen.
  In the spleen, they are localised at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones.
  In lymph nodes, they are localised in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyer's patches they are localised in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1, although antigen expression is weaker than that seen in splenic macrophages.
  No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin).
  In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
  Negative Species: Human and Rat.
• Formulation: 0.2M Tris/HCl, pH 7.4 and 5-10% Foetal Calf Serum
  State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Database Link:
  Entrez Gene 20612 Mouse
• Background: The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Synonyms: Sialoadhesin, Siglec-1