MHC Class II I-Ab (monomorphic) Rat Monoclonal Antibody [Clone ID: IBL-3/5]

CAT#: SM078

MHC Class II I-Ab (monomorphic) rat monoclonal antibody, clone IBL-3/5, Supernatant

Specifications

Product Data

• Clone Name: IBL-3/5
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 50 µl of 1/5 diluted antibodyto label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: Neat.
  The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
  
• Reactivities: Mouse
• Host: Rat
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: In vivo stimulated, purified mouse B cells.
  Spleen cells from an immunised Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
• Specificity: This antibody recognizes a monomorphic epitope present on murine MHC class II molecules, which are expressed by dendritic cells, B-cells and macrophages.
  This clone is reported to induce homotypic clustering of B cells in vitro.
  Negative Species: Human, Rat.
• Formulation: 0.2M Tris/HCl pH7.4 containing 5-10% Foetal Calf Serum
  State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
• Synonyms: H2-Eb1, H-2 class II histocompatibility antigen I-A beta chain