MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

CAT#: SM084B

MHC Class II RT1B (monomorphic) mouse monoclonal antibody, clone OX-6, Biotin

Specifications

Product Data

• Clone Name: OX-6
• Applications: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 106 cells in 100 µl (This product is routinely tested in flow cytometry on rat splenocytes). This clone has also been described to react with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (4). Other applications not tested. Optimal dilutions should be determined by the user.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat Thymocyte membrane glycoprotein.
  
  Format: This antibody was lyophilised from PBS buffer pH 7.4 with 1.0% BSA as stabilizer. This purified immunoglobulin fraction is conjugated to Biotin. Reconstitute with 1 ml distilled water. The addition of 0.1% sodium azide is recommended after reconstitution.
• Specificity: MRC OX-6 recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region (1).
  
  Crossreactivity: Mouse haplotypes k and s (2). Does not recognise BDIX rat strain (6).
• Formulation: Label: Biotin
• Concentration: IgG concentration 0.09 mg/ml prior to lyophil.
• Conjugation: Biotin
• Synonyms: HLA Class II, MHC Class 2 RT1B