MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

CAT#: SM084P

MHC Class II RT1B (monomorphic) mouse monoclonal antibody, clone OX-6, Purified

Specifications

Product Data

• Clone Name: OX-6
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry (1:50 - 1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
  This product is routinely tested in flow cytometry on rat splenocytes.
  Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100):
  this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4).
  This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6.
  Western blot.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoproteins
• Specificity: This antibody recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.
  It cross reacts with certain mouse strains of MHC haplotypes k and s.
  Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region (1).
  The clone does not react with Rat BDIX strain.
• Formulation: PBS, pH 7.2, containing 0.09% sodium azide
  State: Purified
  State: Liquid IgG fraction
• Concentration: 1.0 mg/ml
• Purification: Protein G affinity chromatography
• Synonyms: HLA Class II, MHC Class 2 RT1B