MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

CAT#: SM084PX

MHC Class II RT1B (monomorphic) mouse monoclonal antibody, clone OX-6, Purified

Product Images

Staining of rat spleen cells with mouse anti rat RT1B

Specifications

Product Data

• Clone Name: OX-6
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemsitry on Paraffin Sections: 1/50-1/100. This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.
  This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoprotein.
  Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
• Specificity: This antibody recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.
  This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This antibody does not react with the BDIX rat strain. Expression is polymorphic in mice.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Synonyms: HLA Class II, MHC Class 2 RT1B