T Cell Receptor (TCR) gamma/delta Hamster Monoclonal Antibody [Clone ID: GL3]

CAT#: SM093P

T Cell Receptor (TCR) gamma/delta hamster monoclonal antibody, clone GL3, Purified

Specifications

Product Data

• Clone Name: GL3
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/5-1/50 diluted antibody to label 10e6 cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: C57BL/6J intra epithelial lymphocytes.
• Specificity: Reacts with TCR gamma/delta expressing lymphocytes.
  Clone GL3 has been shown to have depleting activity in vivo (2,3).
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography
• Background: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level.
  The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. It is expressed during thymopoiesis and on the small subpopulation of gamma/delta TCR expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma/delta+ and have limited receptor diversity. The TCR gamma/delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation.
• Synonyms: TCRG, TCRD, T-Cell Receptor gamma, T-Cell Receptor delta, T-Cell Receptor gamma delta