Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: Mc24-IC6]

CAT#: SM1009F

Human Lambda Light Chain mouse monoclonal antibody, clone Mc24-IC6, FITC

Specifications

Product Data

• Clone Name: Mc24-IC6
• Applications: FC
• Recommended Dilution: Flow cytometry: use neat antibody to label 10e6 cells or 10 µl washed whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
• Specificity: This antibody recognises lambda light chains, and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein A
• Conjugation: FITC
• Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).