Cd19 Rat Monoclonal Antibody [Clone ID: 1D3]

CAT#: SM1050P

Cd19 rat monoclonal antibody, clone 1D3, Purified

Product Images

Surface staining of CD19 in murine splenocytes with anti-CD19 (1D3) PE.

Specifications

Product Data

• Clone Name: 1D3
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Immunohistochemistry (frozen sections).
  Functional application: This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse CD19-transfected cell line
• Specificity: This antibody detects CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
• Formulation: Phosphate buffered saline (PBS)
  State: Purified
  State: Liquid Ig fraction
  Preservative: 15 mM sodium azide, approx. pH 7.4
• Concentration: 1.0 mg/ml
• Purification: Protein-G affinity chromatography (> 95% pure by SDS-PAGE)
• Database Link:
  Entrez Gene 12478 Mouse
• Background: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
• Synonyms: Leu-12, B-cell marker